TLR2의 활성이 MDPC-23 세포의 부착과 이동에 미치는 영향
TLR2 Activation Promotes Adhesion and Migration of Murine Dental Papilla Derived MDPC-23 Cells
안미영, 신동은, 권성민, 안지위, 이준, 윤정훈,
소속 상세정보
안미영 ( Ahn Mee-Young ) - 원광대학교 치과대학 대전치과병원 구강병리과
신동은 ( Shin Dong-Eun ) - 원광대학교 치과대학 대전치과병원 구강병리과
권성민 ( Kwon Seong-Min ) - 원광대학교 치과대학 대전치과병원 구강병리과
안지위 ( Ahn Ji-Wee ) - 원광대학교 치과대학 대전치과병원 구강병리과
이준 ( Lee Jun ) - 원광대학교 치과대학 대전치과병원 구강악안면외과
윤정훈 ( Yoon Jung-Hoon ) - 원광대학교 치과대학 대전치과병원 구강병리과
KMID : 0829220130370020051
Abstract
Odontoblasts and/or dental pulp cells are responsible for tooth repair as well as dentin formation. Adhesion and migration are critical processes for tissue regeneration. This study was performed to clarify whether Pam3 modulates adhesion and migration of a murine odontoblast-like cell line, MDPC-23 cell and TLR2 signaling is engaged in this process. TLR2 expression in MDPC-23 cells was examined by RT-PCR. Adhesion assay was performed using type Ⅰ collagen-coated plates. Migration ability was determined by a commercial assay kit. Phosphorylation of IκB-α, JNK, p38, and ERK was examined by Western blot analysis. TLR2 was functionally expressed in MDPC-23 cells. Pam3CSK treatment enhanced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Blockade of TLR2 using its antibody restored Pam3CSK-induced adhesion and migration of MDPC-23 cells. These findings indicate that Pam3CSK, an immune activator from gram negative bacteria, can promote adhesion and migration ability of MDPC-23 cells via TLR2.
키워드
TLR2; Odontoblast; Adhesion; Migration; Dentinogenesis
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